Fig. 3
From: Inositol 1, 4, 5-trisphosphate receptor interacts with the SNARE domain of syntaxin 1B
Identification of IP3R1-binding region in Syx1B. a Schematic representation of the domain structure of Syx1B and its deletion mutants fused to MBP. N-terminal regulatory domain (Habc, amino acids 1–143), the linker region (amino acids 144–188), the SNARE domain (amino acids 189–265), and the single C-terminal transmembrane domain (TMD, amino acid residues 266–288). The numbers refer to amino acids. b MBP pull-down assays with mouse brain microsomal lysate. The microsomal lysate was incubated with MBP or MBP-fused Syx1B deletion mutants, and the eluates from the amylose resin beads were analyzed by immunoblotting with anti-IP3R1. Immunoblots with short exposure (upper panel) and long exposure (middle panel) using anti-IP3R1, lower panel immunoblot using anti-MBP to detect MBP and MBP-fused Syx1B deletion mutants