Fig. 2

Identification of the Syx1B-binding region in IP₃R1. a Schematic representation of GST-fused IP₃R1 deletion mutants. The various regions of the IP₃R1 were prepared as GST fusion proteins. The numbers refer to amino acids. b, c GST pull-down assays with mouse brain microsomal lysate. The microsomal lysate was incubated with each GST-fusion proteins immobilized on Glutathione–Sepharose 4B beads, and the bound proteins were analyzed by immunoblotting with anti-Syx1B. Upper panels in (b, c) immunoblot using anti-Syx1B; lower panels in (b, c) amido black staining indicating GST and GST-fused IP₃R1 deletion mutants