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Fig. 1 | The Journal of Physiological Sciences

Fig. 1

From: Inositol 1, 4, 5-trisphosphate receptor interacts with the SNARE domain of syntaxin 1B

Fig. 1

Binding of Syx1B with IP3R1N-terminal cytosolic region. a A schematic representation of the domain structure of IP3R1 and its deletion mutant fused to GST (GST-EL). N-terminal IP3-binding domain (amino acids 1–579); the internal coupling domain (amino acids 580–2,217); the C-terminal domain (amino acids 2,218–2,749). The numbers refer to amino acids. b GST pull-down assays with mouse brain microsomal lysate. The microsomal lysate was incubated with GST or GST-EL, and the eluates from the Glutathione–Sepharose 4B beads were analyzed by immunoblotting with anti-Syx1B. Upper panel immunoblot using anti-Syx1B, lower panel amido black staining indicating GST and GST-EL proteins

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