From: Involvement of brain ketone bodies and the noradrenergic pathway in diabetic hyperphagia in rats
Peptide | Gene | Oligonucleotide primer sequences (5′ → 3′) | |
---|---|---|---|
Forward | Reverse | ||
RT-PCR | |||
 MCT1 | Slc 16a1 | TCTGGCTGTGGCTTGATTGCAGCTT | GACTGACAGCTTTTCTCCTTTGGGA |
 Actb | Actb | TGTCACCAACTGGGACGATA | GGGGTGTTGAAGGTCTCAAA |
Real-time PCR | |||
 Npy | Npy | CCATGTGGTGATGGGAAATG | CAACGACAACAAGGGCCCTGG |
 Agrp | Agrp | TTGGCAGAGGTGCTAGATCCA | AGGACTCGTGCAGCCTTACAC |
 Actb | Actb | ATGTGCTGCCTGACGGTCAG | GGAAGGCTGGAAGAGAGCCT |
 | Probe (5′ → 3′) | ||
 Npy | FAM-GTGCCACCACCAGGCTGGATTCC-TAMRA | ||
 Agrp | FAM-CGAGTCTCGTTCTCCGCGTCGC-TAMRA | ||
 Actb | HEX-CATCACTATCGGCAATGAGCGGTTCC-TAMRA |