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Table 1 Primers used in this study

From: Involvement of brain ketone bodies and the noradrenergic pathway in diabetic hyperphagia in rats

Peptide

Gene

Oligonucleotide primer sequences (5′ → 3′)

Forward

Reverse

RT-PCR

 MCT1

Slc 16a1

TCTGGCTGTGGCTTGATTGCAGCTT

GACTGACAGCTTTTCTCCTTTGGGA

 Actb

Actb

TGTCACCAACTGGGACGATA

GGGGTGTTGAAGGTCTCAAA

Real-time PCR

 Npy

Npy

CCATGTGGTGATGGGAAATG

CAACGACAACAAGGGCCCTGG

 Agrp

Agrp

TTGGCAGAGGTGCTAGATCCA

AGGACTCGTGCAGCCTTACAC

 Actb

Actb

ATGTGCTGCCTGACGGTCAG

GGAAGGCTGGAAGAGAGCCT

 

Probe (5′ → 3′)

 Npy

FAM-GTGCCACCACCAGGCTGGATTCC-TAMRA

 Agrp

FAM-CGAGTCTCGTTCTCCGCGTCGC-TAMRA

 Actb

HEX-CATCACTATCGGCAATGAGCGGTTCC-TAMRA