Fig. 1

a Time course of changes in [Ca²⁺]_i in response to Con A (2 μg/ml) in splenic lymphocytes from control and alloxan-induced diabetic mice. b Effects of Con A and Con A plus verapamil on [Ca²⁺]_i in splenic lymphocytes from control (n = 7 per group) and alloxan-induced diabetic (n = 7 per group) mice. Fura-2-loaded lymphocytes were incubated for 8 min with or without Con A. Verapamil was added 10 min before exposure to Con A. The net Δ[Ca²⁺]_i is the difference between the increase in [Ca²⁺]_i at 8 min after addition of Con A or Con A plus verapamil, and the vehicle, respectively. Data express group mean ± SEM (7 independent experiments performed in duplicate). Statistically significant differences are indicated as: **P < 0.0001 versus control group, ^#P < 0.0001 versus corresponding Con A (2 μg/ml) group