Fig. 3
Hypoxia-induced down-regulation of Kv2.1 channel expression in cultured rat PASMCs and PA were partly restored by 15-LOX inhibitor CDC (5 μM) under hypoxia. a Western blotting of Kv2.1 channel expression in PASMCs. b Densitometric analysis of the western blot (n = 4, **P < 0.01, *P < 0.05). c PCR-amplified products were displayed in agarose gels stained with ethidium bromide for Kv2.1 (321 bp) and β-actin (500 bp) transcripts. Lane 1 Marker, Lane 2 control, Lane 3 hypoxia, Lane 4 hypoxia + CDC, Lane 5 hypoxia + CDC + 15-HETE, Lane 6 hypoxia + 15-HETE. d Densitometric analysis of the RT-PCR (n = 4, **P < 0.01, *P < 0.05). e Western blotting of Kv2.1 channel expression in cultured rat PA. f Densitometric analysis of the western blot (n = 4, **P < 0.01, *P < 0.05). g PCR-amplified products were displayed in agarose gels stained with ethidium bromide for Kv2.1 (321 bp) and β-actin (500 bp) transcripts. Lane 1 Marker, Lane 2 control, Lane 3 hypoxia, Lane 4 hypoxia + CDC, Lane 5 hypoxia + CDC + 15-HETE, Lane 6 hypoxia + 15-HETE. h Densitometric analysis of the RT-PCR (n = 4, **P < 0.01, *P < 0.05)