Fig. 2

Hypoxia-induced down-regulation of Kv2.1 channel in cultured rat pulmonary artery was partly restored by 15-LOX inhibitors CDC (5 μM) and NGDA (50 μM). a Western blotting of Kv2.1 channel. b Densitometric analysis of the western blot (n = 4, **P < 0.01, *P < 0.05). c PCR-amplified products were displayed in agarose gels stained with ethidium bromide for Kv2.1 channel (321 bp) and β-actin (500 bp) transcripts. Lane 1 Marker, Lane 2 control, Lane 3 hypoxia, Lane 4 hypoxia + CDC, Lane 5 hypoxia + NDGA. d Densitometric analysis of the RT-PCR. (n = 4, **P < 0.01, *P < 0.05)