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Fig. 6 | The Journal of Physiological Sciences

Fig. 6

From: Skeletal muscle expression of bone morphogenetic protein-1 and tolloid-like-1 extracellular proteases in different fiber types and in response to unloading, food deprivation and differentiation

Fig. 6

TLL1 promoter and mutagenesis. a Sequence of ~1,300 bp of the mouse TLL1 upstream promoter region. The 40-bp Serum response factor, Agamous, deficiens (SMAD)/CAGA repeat, the forkhead box O (FoxO)-like sequence (TATTTATCTG; on the minus strand), and the nuclear factor-kappa beta (NF-kappaB) consensus (GGGAGCTTCC). b TLL1 promoter-reporter activity in undifferentiated C2C12 myoblasts and 2-day differentiated C2C12 myotubes. Differentiation was associated with a significant decrease in TLL1 promoter activity. Asterisk significantly different from MB, p < 0.05. Results are reported as mean ± SEM of mRNA levels normalized to those of myoblasts. Asterisk significantly different from myoblasts, p < 0.05. c Wild-type (WT) and mutated/deleted TLL1 promoter–reporter activity in differentiated C2C12 myotubes. Deletion of the 40 bp CAGA repeat or mutation of the NF-kappaB site significantly decreased TLL1 promoter activity relative to wild-type. Results are reported as mean ± SEM of mRNA levels normalized to those of the wild type construct. Asterisk significantly different from wild-type, p < 0.05. See Fig. 1 for other abbreviations used

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