Fig. 2

Immunohistochemical localization of BMP-1 in mouse calf muscles. a Low power image of BMP-1 (red) and laminin (green) immunofluorescence staining showing the relative lack of BMP-1-positive fibers in the superficial portion of the calf. b Low power image of BMP-1 (red) and laminin (green) immunofluorescence staining showing the fiber-specific pattern with more BMP-1-positive fibers in the deep portion of the calf. G, P, and S show the relative locations of the gastrocnemius, plantaris, and soleus muscles, respectively. Scale bar in (b) is 0.5 mm and is the same for (a). c High power image of BMP-1 (red) and type I MyHC and laminin (green) immunofluorescence staining of the SOL showing the co-localization of BMP-1 with MyHC type I. Asterisks indicate fibers positive for both BMP-1 and type I MyHC, arrowheads fibers positive for BMP-1 but negative for type I MyHC. d High power image of BMP-1 (red) and type IIa MyHC and laminin (green) immunofluorescence staining showing the co-localization of BMP-1 with MyHC type IIa in the SOL. Asterisk indicate fibers positive for both BMP-1 and type IIa MyHC, arrowheads fibers positive for BMP-1 but negative for type IIa MyHC. Scale bar in (d) is 0.1 mm and is the same for (c) and (d). e, f Serial sections with BMP-1 (red) and type IIb MyHC and laminin (green) immunofluorescence staining showing the lack of co-localization of BMP-1 and MyHC type IIb. Arrowheads indicate fibers positive for BMP-1 but negative for type IIb MyHC in (e). Scale bar in (f) is 0.1 mm and is the same for (e) and (f). See Fig. 1 for the abbreviations used (color figure online)