A stable ATP binding to the nucleotide binding domain is important for reliable gating cycle in an ABC transporter CFTR

Table 2 Parameter sets (s⁻¹) for the “stay and stray” hypothesis

	k_CO1	k_O1C	k_O1O3	k_O3O1
Y1219G	3 or 0^a	3	2 × 10⁻²	3.8 × 10⁻²
Y1219I	↑	↑	1.3 × 10⁻²	3.8 × 10⁻²
Y1219F	↑	↑	2 × 10⁻²	7 × 10⁻²
Y1219W	↑	↑	1 × 10⁻²	1 × 10⁻¹

^ak_CO1 was set to 3 s⁻¹ for simulating the “with ATP” condition and 0 s⁻¹ for simulating the “after the ATP washout” condition. Note that we employed the common values for k_CO1 in all the Y1219 mutants whereas they should actually have different k_CO1 values [21], because it does not affect the reproduced current relaxation

ISSN: 1880-6562