Fig. 2
Expression patterns of voltage-gated Ca2+ channel α1 subunits in male and female rat GnRH neurons. The appearance of positive bands in the RT-PCR analyses is shown as a percentage of the total reactions for each subunit. a, b Results were obtained with GnRH neurons harvested from DBB/OVLT slices and mPOA slices. The numbers of reactions are indicated in the figures. c Expression patterns of α1 subunits for T-type Ca2+ channel in estrous cycle stages and males. D diestrus (n = 21), P proestrus (n = 18), E estrus (n = 18), males (n = 18). Cells were harvested from an acutely dispersed preparation