Fig. 1

Multi-cell RT-PCR analyses of mRNAs encoding voltage-gated Ca²⁺ channel α₁ subunits in adult rat GnRH neurons. Cytosols harvested from five GnRH neurons in slice preparation were pooled and reverse-transcribed to generate cDNAs, which were then subjected to PCR amplification with specific primers. The amount of cDNA used for each PCR corresponds to one cell for the Ca²⁺ channel α₁ subunits and 0.2 cells for GnRH. M 100-bp marker ladder, N cytosols without reverse transcriptase, R reference tissues (hypothalamus for α_1A–E, α_1G–H, and GnRH; skeletal muscle for α_1S; eye for α_lF)