Fig. 4
Transcellular calcium transport in TRPV5/TRPV6, TRPV5/Cav1.3, TRPV6/Cav1.3 double KD, and TRPV5/TRPV6/Cav1.3 triple KD monolayers directly exposed to 600 ng/mL rhPRL. PEI was a transfecting agent. Monolayer was bathed on both sides with 1.25 mmol/L calcium-containing solution. ***P < 0.001 compared with the scrambled siRNA-treated group (negative control). Numbers in parentheses represent the number of independent Snapwells. siTRPV5, siTRPV6, and siCav1.3 mean siRNAs against TRPV5, TRPV6, and Cav1.3 mRNA, respectively. NS Not significant