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Fig. 1 | The Journal of Physiological Sciences

Fig. 1

From: Transepithelial calcium transport in prolactin-exposed intestine-like Caco-2 monolayer after combinatorial knockdown of TRPV5, TRPV6 and Cav1.3

Fig. 1

a Expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA in the control, PEI-treated, scrambled siRNA (Scramble)-transfected and TRPV5 siRNA (siTRPV5)-transfected Caco-2 monolayers. GAPDH expression of the control group was adjusted to 100%, while those of other groups were relative to the control group. b Representative electrophoretic bands of TRPV5, TRPV6 and Cav1.3 expression in the control, PEI, Scramble and siTRPV5 groups. ce Expression of TRPV5, TRPV6 and Cav1.3 in TRPV5 KD Caco-2 monolayer as demonstrated by qRT-PCR. Expression levels were normalized by GAPDH expression. Values are presented as log mean ± SE. ***P < 0.001 compared with the control group. Value of fold difference was also presented along with the statistical symbol. Numbers in parentheses represent the number of independent experiments. Each experiment was performed in triplicate

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