Fig. 2

Retarded extension of cell outgrowth from TGF-beta1 knockdown EBs on gelatin-coated surface. D5 EBs of the R1, LIR1 (pDS_control vector), and TIR1 (TGF-beta1 knockdown) groups were cultured on a gelatin-coated surface for 3 days and were analyzed. a Phase contrast microscopy of differentiated EBs of the three groups. b Quantitative analysis of the outgrowth in EBs of the three groups. c Fluorescence microscopy of yellow fluorescent protein (YFP) and SM alpha-actin of the same cell outgrowth from differentiated EBs of the LIR1 and TIR1 groups. Scale bar 100 μm