Fig. 1

Identification of TGF-beta1 knockdown ES cell clones. a Phase contrast and fluorescence microscopy of ES cell clones of R1 (wild type), TIR1 (TGF-beta1 knockdown), and LIR1 (pDS_control vector). Scale bar 100 μm. b Western blot analysis for TGF-beta1 expression in ES cells and EBs at three stages of differentiation (D5, D10, and D15) of R1, TIR1, and LIR1 with beta-actin as loading control. c Quantification of TGF-beta1 expression in EBs of R1, LIR1, and TIR1 with beta-actin as loading control. **p < 0.01