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Fig. 3 | The Journal of Physiological Sciences

Fig. 3

From: Senescent endothelial progenitor cells from dogs with pulmonary arterial hypertension: a before–after self-controlled study

Fig. 3

CD133/KDR-positive cells were detected in peripheral blood and identified as circulating EPCs by flow cytometry using counting beads tubes. a Gated and counted beads from a fluorescein isothiocyanate (FITC) versus side-scattering corner (SSC) dot plot. Quadrants were set on the basis of isotype-identical controls. b Dual-positive CD133/KDR cells were identified and numerated in the upper right quadrant of the scatter plot from blood of dogs. The representative dot plots are represented. The absolute counts of positive cells per ml blood were calculated using the equation described in “Methods”. c Comparison of circulating EPCs number before and after PAH. d Differentiated EPCs were significantly reduced in PAH but transiently raised at the early stage after DHMC. Data are mean ± SD. *p < 0.05 versus baseline, # p < 0.05 versus 48 h

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