Fig. 7
E2-induced ER increase involves ERK1/2 and p38 pathways. Histograms indicate the relative density of ER immunoblots normalized to control (basal level). a From left to right, vehicle, E2 10−8, PD9809 alone, and E2 + PD98059. b Time course of ERK phosphorylation induced by E2. ERK phosphorylation assessed by pERK-specific antibody. c From left to right, vehicle, E2 (10−8 M), SB203580 (10 μM) alone, and E2 + SB203580. In a and c, PD98059 (10 μM) or SB203580 (10 μM) was added into the culture medium 30 min before addition of E2 (10−8 M). The bars show means ± SE of three independent experiments performed in duplicate. *P < 0.01, compared with control values, determined using Student’s t test. ☆ P < 0.01, compared with E2 values, determined using Student’s t test