Fig. 2

Dose-dependent and time-dependent NO release from NE-MD cells pre-incubated with furosemide. a (Dose-dependent): NE-MD cells were incubated in the presence of furosemide for 5 h. The NO values are 1.6 ± 1.3 (0 mM l-Arg (control), n = 4), 6.5 ± 2.7 (0.2 mM l-Arg, n = 4), and 79.7 ± 24.1 nM (1 mM l-Arg, n = 4). b (Time-dependent): l-Arg (1 mM)-induced NO release from the pre-treated NE-MD cells: 4.6 ± 3.0 (0 h (control), n = 3), 56.0 ± 16.6 (2 h, n = 4), and 79.7 ± 24.1 nM (5 h, n = 4). * and ** are P < 0.05 and P < 0.02, respectively, compared with the values of control; ns not significant