Fig. 6
From: Involvement of aquaporin-5 in differentiation of human gastric cancer cells
HgCl2-induced inhibition of the cell differentiation in the AQP5-expressing cells. a Western blotting for checking expression of AQP5 (27 kDa) in the mock cells (control cells; cont) and AQP5-expressing cells [stable expression of AQP5; AQP5(S)]. As a loading control, expression level of β-actin (42 kDa) was tested. b, c The number of differentiated cells in 100 cells stably expressing AQP5 (b) and in 100 control cells that do not express AQP5 (c). n = 8–10. **Significantly different versus zero HgCl2 (p < 0.01). NS not significant versus zero HgCl2 (p > 0.05). d, e The total cell number in the AQP5-expressing cell well (d) and control cell well (e). In each well, 1 × 105 cells were seeded. n = 3. **p < 0.01; NS, p > 0.05. f, g Alkaline phosphatase (AP) activity in the AQP5-expressing cells (f) and control cells (g). n = 3. **p < 0.01; NS, p > 0.05