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Fig. 4 | The Journal of Physiological Sciences

Fig. 4

From: Involvement of aquaporin-5 in differentiation of human gastric cancer cells

Fig. 4

Cancer cell differentiation induced by sodium butyrate or expression of AQP5. a, b MKN45 cells were cultured in the presence [SB(+)] and absence [SB(−)] of 2 mM sodium butyrate for 48 h. Sodium butyrate was added at 24 h after the cell seeding. In a, representative images are shown. Differentiated cells (spindle-shaped) are marked by arrows. In b, the number of differentiated cells in 100 cells in the presence and absence of SB are shown. n = 20. **p < 0.01. cf The cells were transfected with the AQP5-pcDNA4/His vector (AQP5) or pcDNA4/His vector (mock) at 24 h after the cell seeding and cultured furthermore for 48 h. In c, representative images are shown. In d, the number of differentiated cells in 100 cells transfected with mock and AQP5 are shown. n = 20. **p < 0.01. In e, alkaline phosphatase (AP) activities in the AQP5-transfected cells and mock cells are shown. n = 6. **p < 0.01. In f, the total cell numbers in the AQP5-transfected cell and mock cell wells are shown. The number of cells in a 12-well culture plate was assessed. In each well, 1 × 105 cells were seeded. n = 6. *p < 0.05

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