Single-channel recordings of maxi-anion channel currents in on-cell patches activated by osmotic swelling of mammary C127 cells and in inside-out patches excised from the cells. a Representative current traces recorded under isotonic and hypotonic conditions on C127 cells during application of alternating pulses from 0 to ±25 mV (protocol is shown at the top of the traces). b Unitary I–V relationships for the single-channel events recorded in on-cell patches (open circles) and in inside-out patches (filled circles). Each symbol represents the mean ± SEM (vertical bar). Modified from Sabirov et al.