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Table 1 Primers used for 3′-, 5′-RACE, ORF cloning, and plasmid construction

From: Gene structures, biochemical characterization and distribution of rat melatonin receptors

Gene Direction Primer sequences
3′-RACE
 Universal
  RT primer Reverse 5′-GCTGTCAACGATACGCTACGTAACGGCATGACAGTG(T)18-3′
  1st PCR Reverse 5′-GCTGTCAACGATACGCTACGTAACG-3′
  2nd PCR Reverse 5′-CGCTACGTAACGGCATGACAGTG-3′
 MT1
  1st PCR Forward 5′-AAACCGGACAGCAAACCCAAACT-3′
  2nd PCR Forward 5′-GGGCCCCACTCAACTTCATAGGTC-3′
 MT2
  1st PCR Forward 5′-CCGAAGGAAGGCAAAGGCTGAGAG-3′
  2nd PCR Forward 5′-CCAGAAGGGCTTTTTGTCACCAG-3′
5′-RACE
 MT1
  RT primer Reverse 5′-pAGTAACTAGCCACGAAGA-3′
  1st PCR Forward 5′-AAACCGGACAGCAAACCCAAACT-3′
Reverse 5′-GCCCAGGATGTCCACCACGATAGT-3′
  2nd PCR Forward 5′-GGGCCCCACTCAACTTCATAGGTC-3′
Reverse 5′-ACGACGGCCGCGATCTTATTTC-3′
 MT2
  RT primer Reverse 5′-pATTAAGGCAGCTGTTGAA-3′
  1st PCR Forward 5′-CCGAAGGAAGGCAAAGGCTGAGAG-3′
Reverse 5′-GGTGCTGGCTGTCTGGATGAAGGT-3′
  2nd PCR Forward 5′-CCAGAAGGGCTTTTTGTCACCAG-3′
Reverse 5′-TGACTGCAGGCTCGGTGGTAGGT-3′
ORF cloning
 MT1 Forward 5′-GCGCGGGGCTACAGGATGATG-3′
Reverse 5′-CAGGAGGTCCGCTCCAACACTATG-3′
 MT2 Forward 5′-GGAGCGCCCCCAAGCAGT-3′
Reverse 5′-AGGTCAAGGTGGCAGGGAAAATG-3′
Plasmid construction
 MT1 Forward 5′-CACCATGAAGGGCAATGTCAGC-3′
Reverse 5′-TTAAACAGAGTCCACCTTTATTAAATT-3′
 MT2 Forward 5′-CACCATGCCTGACAACAGCTCCAT-3′
Reverse 5′-CTAGAGAGCACCTTCCTGGACAG-3′
  1. To design the primers for cloning of rat MT1 and MT2, we referred to AF130341 for MT1 and AF141863 for MT2